The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. com. Published Dec 16, 2020 | Updated Dec 16, 2020. Infinite possibilities. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. Optimized for fluorescence microplate readers. d. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Other. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. Three low volume 24-well bottom slides. 9. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Published Dec 16, 2020 | Updated Dec 16, 2020. , Corning, NY) or Greiner. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Versa Max. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. With various on-board features and capabilities, the ThermoMax. All trademarks used herein are the property of. 96-, 384-, and 1536-well microplates. Gemini EM Reader scans. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 5in (46. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. No tools required to assemble. May require LS-50B…. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Integrated stacker option. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Intuitive graphical user interface makes each assay extremely easy to set. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Service Contracts, Preventative Maintenance and. More Product Information. REF (SpectraMax. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. 1 OD at 230 nm. The plate reader optics are tunable for. Time-resolved phosphorescence. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. UV absorbance detection. Category 2 and 3 microplate readers use gradient heating as well. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. #N207) LAL Reagent Water (Lonza cat. Important things to consider when purchasing a multimode microplate reader. The picked. 0025 OD from 0. minutes and begin reading microplates with seven mouse clicks. No. Populate the following info under Description. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Plus 384. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5% at 1. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. 20 attomol ATP per well. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Please click on the links below to view the Microplate Reader Comparison Tables. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. High-throughput, automated dynamic light scattering. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Step 3: Click “Settings” and a screen will appear (Figure 1). Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. x. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Next-generation luminescence plate reader. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. Figure 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 000 ABS • Resolution: 0. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Expand Microplates and select the newly created plate. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 00. *For Corning UV plates, mean values at 260 nm ranged from 0. Categories: Plate Readers. Quantitative, whole well cell confluence for every well. 5. a white 96-well half-area microplate (Corning cat. Fluorescence Microplate Reader Comparison Chart. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. VIS absorbance detection. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 00. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Dimensions: 18. #ABS Plus) Cell plating and treatment prior to assay. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. Loading Buffer was also added to a set of cell-free wells for background controls. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. A. Reconstitution of. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Site. Luminescence applications and assays. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. 96 well plate injection time with SpectraMax® L plate reader. Top or bottom detection is available with a simple click of a button. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Compact designs make these instruments. Linear, Orbital Slow, and Orbital Fast. It offers extremely low absorbance background, excellent. $488. They are also sealable using different types of adhesive mats foils or films. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. Use standard plate unless you see your type listed. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. Some will only support one well density (e. Published Dec 16, 2020 | Updated Jun 22, 2023. QC tools and IQOQ services. Range of 96, 384-well SBS microplates . During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Therefore you need to ask a physicist. • Remove media from each well using a blue pipette tip being careful as not to remove cells. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. These lab workhorses are used in drug discovery. 00 . If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. 4 (Molecular Devices cat. x. Description. High tolerance for contaminants. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Return to top. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Unparalleled performance on a personalized platform. We would like to show you a description here but the site won’t allow us. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. 305 reference level saturation. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Plate Shaking. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. A = Log 10 (I 0 /I). luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Reader Documentation Version No. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Optional 12-way manifold) 5032336. Cell viability and cytoxicity assays. 5°C at 37°C. robotics-automation-compatible. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 2 OD at 200 nm; this decreased to 0. This can increase throughput and efficiency, thus saving time and space in the lab. 4 (Molecular Devices cat. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 2% in. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. In SoftMax Pro, Dual Read mode was selected. Artel MVS Bundle DM Std/800TSNB Reader Details. The color touch screen provides a visual user interface, making programming fast and intuitive. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Controlled by the computer. Corning. 1 mL/well of media. Consequently, FLIPR has been used. Industry standard, 96-well plates. 1% in white 96- and < 0. 500 OD ± 3. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. 1 x plates; Instrument dimensions. 00. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Microplate Capacity. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Two different agonists, acetylcholine and carbachol, were tested. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. $3,207. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. UV absorbance detection. Step 2: Launch SoftMax Pro Software. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. SpectraMax i3x Multi-Mode Microplate Reader. From the dropdown Menu, select Wellplates. 2. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). M2. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. S. 96- or 384-well microplate compatibility. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Instrument settings can. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Compare this item. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Temperature control: 4°C above ambient to 45°C. A standard 96 well plate was set up as described in Protocol Table 4. 96- and 384-well microplates. Gemini XPS Reader scans. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Growth medium was then replaced with fatty acid uptake solution. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. adjust the reader. Find our technical support information along with contact details. 3) At the bottom of the strip, click on "Show. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. These robust readers have been placed in labs from Antarctica to the. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Step 1: Capture antibody binds to ELISA plate wells. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. #E010) Pyroplate 96-well microplate (ACC cat. These models can no longer be repaired. Figure 1-2 Match chamfered corners of slide and adapter 2. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. This reader can also be used as a standard. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Four wash/rinse bottles are included and configurable for both 96- and 384. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Microplate Readers. 313 reference gain check fail. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. (Molecular Devices cat. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. p-Nitroaniline - Catalog No. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. x. x. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. . Absorbance Linearity: 2. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. UV Plate 96 Well, Flat Bottom, Cs 50. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 0, or. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Dark blue indicates well will be read. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. Place the microplate in the drawer. The procedures in this protocol support the 96-well reaction plate. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. 96-well) while others have flexibility to. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Packed 25 plates per…. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Microplate readers, SpectraMax®, with SpectraDrop. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Part of a plate can be selected so that only a selected area of the plate. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 96- and 384-well. Corning. 96-, 384-, and 1536-well microplates. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Automated live cell imaging – cell counting and confluence. x. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. 25, 3. Cayman Chemical. Products : Ionworks HT, Ionworks Quattro. Shaking: Linear, orbital, and double orbital. minutes and begin reading microplates with seven mouse clicks. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 046 to 0. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. U-bottom: 30-40 µl. with Quartz ABS Plastic. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. 1. Fluorescence Microplate Reader Comparison Chart. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. FlexIII. Polypropylene deep well plates, round bottom. Materials. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Detection of KASP genotyping reactions. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Uncontrolled Copy for Training and Reference Purposes Only . BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. The Versamax Microplate Reader is built around a grating monochromator. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. (one set per assay) to clear 96-well microplates. President of Molecular Devices. to each well. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time.